Structured Illumination Microscopy Image Reconstruction Algorithm
نویسندگان
چکیده
منابع مشابه
Plasmonic structured illumination microscopy.
We propose a super resolution imaging technique called plasmonic structured illumination microscopy (PSIM), which combines the structured illumination microscopy technique with the tunable surface plasmon interference. Because of the high-resolution enabled by using surface plasmon interference as an illumination source, PSIM possesses higher image resolving power compared with conventional str...
متن کاملBlind Structured Illumination Microscopy Reconstruction in Thick Samples
Aurélie Jost, Elen Tolstik, Bo-Jui Chang, Ernst H.K. Stelzer, Anne Sentenac and Rainer Heintzmann Institute of Physical Chemistry, Abbe Center of Photonics, Friedrich-SchillerUniversity Jena, Helmholtzweg 4, 07743 Jena, Germany Leibniz Institute of Photonic Technology, Albert-Einstein Str. 9, 07745 Jena, Germany BMLS, Physical Biology, Goethe University, D-60438 Frankfurt am Main, Germany Insti...
متن کاملA joint Richardson—Lucy deconvolution algorithm for the reconstruction of multifocal structured illumination microscopy data
We demonstrate the reconstruction of images obtained by multifocal structured illumination microscopy, MSIM, using a joint Richardson–Lucy, jRL-MSIM, deconvolution algorithm, which is based on an underlying widefield image-formation model. The method is efficient in the suppression of out-of-focus light and greatly improves image contrast and resolution. Furthermore, it is particularly well sui...
متن کاملQSIM: quantitative structured illumination microscopy image processing in ImageJ
BACKGROUND Structured illumination microscopy has been extensively used in biological imaging due to its low cost and easy implementation. However, the lack of quantitative imaging capability limits its application in absolute irradiance measurements. METHOD We have developed a quantitative structured illumination microscopy image processing algorithm (QSIM) as a plugin for the widely used Im...
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We present a theoretical analysis of the image formation in structured illumination wide-field fluorescence microscopy (SIWFFM). We show that the optically sectioned images obtained with this approach possess the optical sectioning strengths comparable to those obtained with the confocal microscope. We further show that the transfer function behaviour is directly comparable to that of the true ...
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ژورنال
عنوان ژورنال: IEEE Journal of Selected Topics in Quantum Electronics
سال: 2016
ISSN: 1077-260X,1558-4542
DOI: 10.1109/jstqe.2016.2521542